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Material Safety Data Sheet (MSDS) Brefeldin A Solution 1000X     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Brefeldin A Solution (1,000X)
2703005 1 ml $105.00      
Formulation: Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium.
Storage & Handling: Store the Brefeldin A Solution (1,000X) at 4°C
Application:

ICFC - Quality tested
Recommended Usage:

Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.
Application
References:

1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
2. Fuse S, et al. 2007. J. Immunol. 178:5227.
3. Kang YJ, et al. 2007. Nature Immunol. 8:601.
4. Redfern CH,et al.2006.J. Clin Oncol.19:3107.PubMed
5. Smithey MJ, et al. 2008. J. Immunol. 180:3406. PubMed
6. Elzey BD, et al. 2008. Blood 111:38684. PubMed
7. Nguyen KD, et al. 2008. J. Immunol. 181:5386. PubMed
8. Goodridge HS, et al. 2009. J. Immunol. 182:1146. PubMed
9. Markey KA, et al. 2009. Blood 113:5644. PubMed
10. Wilcox RA, et al. 2009. Blood PubMed
11. Zenaro E, et al. 2009. J. Leukoc Bio. PubMed
12. Butcher JP, et al. 2010. Clin Cancer Res. 16:2065. PubMed
13. Boisvert M, et al. 2010. J. Virol. 84:7782. PubMed
14. Liu WC, et al. 2010. J. Virol. 84:12011. PubMed
15. Tawara I, et al. 2011. Clin Cancer Res. 17:77. PubMed
16. Iwata Y, et al. 2011. Blood 117:530. PubMed
17. Schmidt NW, et al. 2011. J. Immunol. 186:3836. PubMed


Description:

Brefeldin A (BFA) is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.
Other Names: BFA
Antigen
References:

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.